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Determination of unauthorized fat-soluble azo dyes in spices by HPTLC
Summary
Type: TLCApplication #: 403660
Detection:
Conditions: ample application: bandwise with automatic TLC sampler ATS 4 (CAMAG, Switzerland), band length 7 mm, track distance 10 mm, distance from lower plate edge 8 mm, distance from left and right edges min. 15 mm Applied volume: 1–20 µL for standard solutions, overspotted by 10 µL internal standard solution, 2 µL for sample extract Development: in automatic developing chamber ADC 2 (CAMAG, Switzerland) with eluent iso-hexane – methyl ethyl ketone (5:1, v/v), tank saturation 10 min, migration distance 68 mm, humidity control by saturated potassium carbonate solution (45 % relative air humidity) for 4 min Documentation: in documentation system DigiStore 2 (CAMAG, Switzerland) under white light illumination
Authors: Schwack, W.; Pellissier, E.
Source: Lebensmittelchemie 64 (2010), 159–160
Details
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