403450 HPTLC Determination of Caffeine in Beverage by Use of the ChromImage Flatbed Scanner

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HPTLC Determination of Caffeine in Beverage by Use of the ChromImage Flatbed Scanner

Summary

Type: TLC
Application #: 403450
Catalog#: 4214137 - Nano-Silica XHL HPTLC Plates, w/UV254, glass backed, 200um, 10x20cm, 50/pk
Detection: Scanning and quantification with ChromImage (AR2i Company), 254 nm
Conditions: Prewashing: The HPTLC plate was developed to the top with 1:1 dichloromethane-methanol and dried.
Applying: 2.0, 4.0, 6.0, 8.0 μl caffeine standard solution and 4.0 μl sample solution
Drying: 5 min at 100° C on a plate heater (Camag)
Development: The plate was predeveloped with methanol to a position 1 cm above the origin, dried for 2-3 min at 100° C on the plate heater, then developed to a distance of 6 cm with ethyl acetate-methanol, 19:1 and dried again.
Authors: Halkina T Sherma J
Source: Department of Chemistry Lafayette College Easton P


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