403440 HPTLC Determination of Caffeine in Analgesic Tablets by Use of the ChromImage Flatbed Scanner

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HPTLC Determination of Caffeine in Analgesic Tablets by Use of the ChromImage Flatbed Scanner

Summary

Type: TLC
Application #: 403440
Catalog#: 4214137 - Nano-Silica XHL HPTLC Plates, w/UV254, glass backed, 200um, 10x20cm, 50/pk
Detection: Scanning and quantification with ChromImage (AR2i Company), 254 nm
Conditions: Prewashing: The HPTLC plate was developed to the top with 1:1 dichloromethane-methanol and dried.
Applying: 2.0, 4.0, 6.0, 8.0 μl caffeine standard solution and 6.0 μl sample solution
Development: Plate was developed with ethyl acetate-glacial acetic acid, 95:5 and dried.
Authors: Halkina T Sherma J
Source: Department of Chemistry Lafayette College Easton P


Details


Legend: SF: solvent front O: Origin C: caffeine (Rf 0.27) A: acetaminophen (Rf 0.50) AC: acetylsalicylic acid (Rf 0.55) lanes 1-4: 2.0 - 8.0 µl standard solution lanes 5-6: 6.0 µl duplicate sample solut