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Separation of steroids on ALUGRAM SIL g / UV 254
Summary
Type: TLCApplication #: 402880
Catalog#: 1634026 - Silica G TLC Plates, aluminum backed, 200um, 20x20cm 25/pk
Detection: UV 254 nm, or spray with a 20 % alcoholic solution of sulphuric acid, heat to 120 °C for 10 minutes, then UV 365 nm or daylight
Conditions: Eluent 1: Dichlormethane / diethyl ether / methanol / water 77:15:8:1.2
Eluent 2: Diethyl ether / toluene / 1-butanol 80:5:5, water saturated
Eluent 3: Acetic acid / carbon tetrachloride / heptane 40:30:30
Eluent 4: Butanol / acetic acid / water 60:20:20
sample volume: 2 μl of a 5 mg/2ml sample in methanol or methanol/dichlormethane 1:9
Distance: 15 cm
Authors: Hoebus J Daneels E Roets E Hoogmartens J
Source: J Planar Chromatogr 6 (1993) 269-273
Details
Legend: hRf values Compound Eluent 1 Eluent 2 Eluent 3 Eluent 4 Dexamethasone sodium phosphate 0 0 0 52 Betamethasone sodium phosphate 0 0 0 52 Prednisolone sodium phosphate 0 0 0 40 Pred