401740 Separation of different thyroid gland hormones (thyroxines)

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Separation of different thyroid gland hormones (thyroxines)

Summary

Type: TLC
Application #: 401740
Catalog#: 0314026 - Cellulose 300 TLC Plates, glass backed, 100um, 20x20cm 25/pk
Detection: for SIL-G: dry at 100 ¡C, after cooling spray with little 50% acetic acid and irradiate some minutes with unfiltered UV light (about 254 nm). Iodine containing compounds show weakly violet to brown s
Conditions: Eluent:
A. acetic acid (95%) / benzene* / xylene (60:20:20, v/v)
B. butanol / acetic acid / water (40:10:50, v/v) or (100:10:10, v/v)
C. butanol / methanol / 20% aqueous ammonia solution (80:20:20, v/v)
D. tert. butanol / 2 N aqueous ammonia solution / chloroform (75:14:12,
v/v)
E. 0.5 N aqueous sodium chloride solution

* instead of benzene we recommend the less toxic toluene
Authors: Lederer, M.
Source: J. Chromatogr. 194 (1980) 270


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