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Purity control of six tetracyclines by semi-quantitative TLC with fluorescence detection
Application #: 401720
Catalog #: 1615026
Detection: plates were flushed in a stream of nitrogen, dipped in a 30% solution of liquid paraffin in n-hexane and inspected under UV light at 365 nm; plates were then protected from light by aluminium foil and
Conditions: Eluent: dichloromethane / methanol / water (60:35:5, v/v for chlortetracycline, 59:35:6, v/v for the other tetracyclines)
Migration distance: 15 cm, chamber saturation
Authors: Naidong, W. et al.
Source: J. Planar Chromatog. 7 (1994) 297–300
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