401600 Densitometric determination of gluconic acid and lactobionic acid in pharmaceutical preparations

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Densitometric determination of gluconic acid and lactobionic acid in pharmaceutical preparations

Summary

Type: TLC
Application #: 401600
Catalog#: 1615026 - Silica G TLC Plates, glass backed, 250um, 20x20cm 25/pk
Detection: dip in lead tetraacetate / 2,7-dichlorofluorescein solution in benzene. In-situ fluorescence measurements allow quantitative determination of the sugar acids gluconic acid and lactobionic acid in pha
Conditions: Eluent:
I. isopropanol / ethyl acetate / water / conc. ammonia
(35:20:25:25, v/v)
II. methanol / water / conc. ammonia (70:20:10, v/v)
III. acetone / ethyl acetate / water / conc. ammonia (50:20:20:10, v/v)
Authors: Gübitz, G.
Source: J. Chromatogr. 117 (1976) 337–343


Details


Legend: Left: Chromatogram of a Calcium Sandoz tablet: 1. citric acid, 2. lactobionate, 3. gluconate, 4. sugars Right: Chromatogram of a Ca-C 1000 Sandoz tablet: 1. gluconate, 2. sugars, 3. ascorbic acid, 4.