401570 Planar chromatography of closely related steroids on untreated silanized silica plates

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Planar chromatography of closely related steroids on untreated silanized silica plates

Summary

Type: TLC
Application #: 401570
Catalog#: 2614156 - C18 Silica HPTLC Plates, w/UV254, 50% silanization, glass backed, 200um, 10x10cm, 25/pk
Detection: The HPTLC plate is sprayed with a fresh solution of 0.5% vanillin in sulphuric acid – ethanol (4 : 1, v/v) and then heated to 100 °C for 15 min.
Conditions: Eluent:
1. acetic acid / acetonitrile / water (10 : 30 : 60)
2. acetic acid / methanol / water (10 : 30 : 60)
3. n-hexane / ethyl acetate / acetic acid (60 : 39 : 1)
Migration distance: 6 cm
Sample volume: 0.3 – 0.5 μl of a solution in water / methanol (1 : 1, v/v)
Authors: Lepri, L. et al.
Source: J. Planar Chromatog. 2 (1989) 214–218


Details


Legend: Compound 1 2 3 1 Cortisone 0.47 0.28 0.40 2 Hydrocortisone,Cortisol 0.47 0.25 0.34 3 Tetrahydrocortisone 0.41 0.14 0.25 4 Allo-tetrahydrocortisone 0.41 0.14 0.33 5 Allo-tetrah