401520 Separation of steroids 5b-pregnane-3a,20a-diol 17b-estradiol and estrone by TLC

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Separation of steroids 5b-pregnane-3a,20a-diol 17b-estradiol and estrone by TLC

Summary

Type: TLC
Application #: 401520
Detection: spray with 1% vanillin in conc. sulphuric acid (see chapter visualization reagents) and heat 5 min to 105 °C
Conditions: Eluent: ethyl acetate / cyclohexane (1:1, v/v)
Migration distance: 10 cm in 20 min, ascending, chamber saturation
Authors: TLC department
Source: TLC department


Details


Legend: Lanes: 1. mixture of 2-4 2. 5b-pregnane-3a,20a-diol (Rf = 0.23) 3. 17b-estradiol (Rf = 0.38) 4. estrone (Rf = 0.53)