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Separation of steroids cortexone corticosterone and cortisone
Summary
Type: TLCApplication #: 401480
Catalog#: 2715126 - C18-W Silica TLC Plates, w/UV254, glass backed, 250um, 20x20cm, 25/pk
Detection: Detection: TLC scanner, dual wavelength UV, 254/370nm
Conditions: samples as in a, eluent acetone / water (1:1, v/v), migration distance 6.8 cm in 25 min, ascending
Authors: TLC department
Source: TLC department
Details
Legend: TLC A) 1. cortexone, 2. corticosterone, 3. cortisone; concentration 0.5% each, sample volume 750 nl; eluent methanol / water / acetic acid (60:46:2, v/v), migration distance 7.8 cm in 25 min, ascendin