401370 Detection of shortchain acid-soluble polyphosphates in cultures of brewers yeast

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Detection of shortchain acid-soluble polyphosphates in cultures of brewers yeast

Summary

Type: TLC
Application #: 401370
Catalog#: 1122026 - Cellulose 300 PEI TLC Plates, polyester backed, 100um, 20x20cm 25/pk
Detection: autoradiography or dip plates 1 - 2 min in ammonium molybdate/perchloric acid solution (see chapter visualization reagents). After 7 – 8 min at 85 °C phosphates appear as yellow-green spots on blue ba
Conditions: Eluent: continuous-flow chromatography with different phosphate buffers according to figure
Authors: Guerrini AM
Source: J Chromatogr 189 (1980) 440


Details


Legend: The figure shows autoradiographs of three 1-dimensional chromatographic separations of 32P-labelled polyphosphates on PEI cellulose plates. Plates were developed at pH 3.4 in a) 1.5 M phosphate buff