401360 TLC separation of inorganic oligophosphates and amidophosphates

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TLC separation of inorganic oligophosphates and amidophosphates

Summary

Type: TLC
Application #: 401360
Catalog#: 1324026 - Special Mixed Layer of Silica Gel with Strongly Basic Anion Exchange Resin, polyester backed, 200um, 20x20cm 25pk
Detection: spray 2x with 1 N perchloric acid in water/acetone (1:1) and dry at 50 °C, then spray with ammonium molybdate in nitric acid and finally with SnCl2 in hydrochloric acid solution (for reagent solutions
Conditions: Eluent: buffer pH 5, for separation of amidophosphates 20 ml pyridine + 13.4 ml glacial acetic acid ad 1000 ml dist. water, for separation of oligophosphates as before + 0.25 mol NaCl
Developing time: about 70 min
Authors: Kroschwitz H
Source: Talanta 19 (1972) 695


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