401080 HPTLC separation of diastereomeric di- and tripeptides

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HPTLC separation of diastereomeric di- and tripeptides

Summary

Type: TLC
Application #: 401080
Detection: spray with 1% ninhydrin in pyridine/ glacial acetic acid (5:1, v/v) and heat 5 min to 100 °C
Conditions: Eluent: 1. 1 M acetic acid + 1 M hydrochloric acid in water / methanol (20%) 2. 0.5 M sodium acetate in water / methanol (20%)
Authors: Lepri L
Source: J Chromatogr 265 (1983) 328


Details


Legend: A) TLC impregnated with 4% N-dodecylpyridinum chloride (N-DPC) using eluent 2 1. L-Leu-L-Tyr, 2. D-Leu-L-Tyr, 3. L-Tyr-L-Arg, 4. L-Tyr-D-Arg, m1 = mixture of 1 and 2, m2 = mixture of 3 and 4.