400960 Nucleic acid constituents : 2-dimensional TLC separation of intracellular purine derivatives

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Nucleic acid constituents : 2-dimensional TLC separation of intracellular purine derivatives

Summary

Type: TLC
Application #: 400960
Catalog#: 0422026 - Cellulose 400 TLC Plates, polyester backed, 100um, 20x20cm 25/pk
Detection: autoradiography or UV 254 nm
Conditions: Eluent:
1st dimension: a) methanol (to top of sheet)
b) methanol / n-propanol / 25% aqueous ammonia solution / 1%
aqueous ethylene diamine tetraacetic acid (EDTA) solution
(3:9:2:6, v/v) to middle of sheet, c) as b), but to top of sheet
2nd dimension isobutyric acid / 25% aqueous ammonia solution / 1% aqueous
EDTA solution (200:9:114, v/v) to top of sheet
Authors: Seipel G
Source: J Chromatogr 135 (1977) 485


Details


Legend: O = origin 1. adenine 2. adenosine 3. hypoxanthine 4. inosine 5. guanosine 6. xanthosine 7. xanthine 8. guanine 9. AMP 10. uric acid 11. NAD 12. IMP 13. ADP 14. GMP 15. XMP 16. ATP 17