400750 TLC analysis of neutral lipids in tissues and hair by methylamine O-deacylation and vitride reduction

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TLC analysis of neutral lipids in tissues and hair by methylamine O-deacylation and vitride reduction

Summary

Type: TLC
Application #: 400750
Catalog#: 1624026 - Silica G TLC Plates, polyester backed, 200um, 20x20cm 25/pk
Detection: mercury chloride Schiff reaction for plasmalogens (see M. H. Hack and F. M. Helmy, An Introduction to Comparative, Correlative Histochemical Principles; VEB Gustav Fischer Verlag, Jena 1974, p. 48, 50
Conditions: Eluent: 1. hexane / diethyl ether (30:5, v/v)
2. chloroform / acetone (2:1, v/v)
3. ethyl acetate / 2-propanol / chloroform / methanol / water (5:5:3:2:1.8, v/v)
Authors: Hack M
Source: J Chromatogr 434 (1988) 215


Details


Legend: Chromatogram of control samples (c) and methylamine O-deacylated extracts (x) of guinea pig tissues after development with eluent 3. From left to right c/x pairs from kidney, heart and brain. Visual