-
Contact an Account Manager
Separation of skin lipids by TLC
Summary
Type: TLCApplication #: 400740
Catalog#: 2115126 - Silica Gel HL TLC Plates, w/UV254, glass backed, 250um, 20x20cm 25/pk
Detection: dip plates in acidic 0.3% MnCl2 solution (120 ml methanol + 120 ml water + 8 ml conc. H2SO4), heat 30 min at 120 °C; quantification with TLC scanner, 550 nm
Conditions: Eluent:
a) methanol
b) diethyl ether
c) n-hexane
AMD gradient technique
Migration distance: 8 cm / 210 min
Authors: Zellmer
Source: Martin-Luther-University Halle-Wittenberg (Germany
Details
Legend: 1. Cholesterol sulfate 2. oleic acid 3. ceramide IV 4. ceramide III 5. cholesterol 6. triacyl glycerol 7. cholesteryl oleate