400650 Optimization of separation of lyophilic fractions of hydrobionts by thin layer chromatography

Contact an Account Manager

Optimization of separation of lyophilic fractions of hydrobionts by thin layer chromatography

Summary

Type: TLC
Application #: 400650
Catalog#: 1615126 - Silica G TLC Plates, w/UV254, glass backed, 250um, 20x20cm 25/pk
Detection: Spraying with a 1.6% -solution of KMnO4 in 100 ml H2SO4, conc., followed by heating at approx. 180 °C for 15-20 min. The compounds are detected as dark spots.
Conditions: Eluent: hexane / diethyl ether / glacial acetic acid
(50 : 50 : 1, v/v/v)
Migration distance: 4 – 6 cm
Sample concentration: 10 ug/ml
Authors: Galinka E
Source: J Planar Chromatog 2 (1989) 158-160


Details


Legend: Rf values: 2-Hydroxyeicosanoic acid 0.33 Methyl-2-hydroxyoctadecanoate 0.56 Stearic acid 0.70