400480 Determination of trigonelline and caffeine contents in coffee by normal phase TLC.

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Determination of trigonelline and caffeine contents in coffee by normal phase TLC.

Summary

Type: TLC
Application #: 400480
Catalog#: 1615026 - Silica G TLC Plates, glass backed, 250um, 20x20cm 25/pk
Detection: Detection: scanner: lcaff. = 272 nm, ltrigo. = 265 nm Rf of caffeine: 0.50 } single development Rf of trigonelline: 0.20 The method is suited for quantitative determination of caffeine and trigonel
Conditions: Eluent: ethanol / 25% hydrochloric acid (95:5, v/v)
for caffeine single, for trigonelline one- to threefold development,
separation time 6 h
Authors: Baltes W
Source: Z Lebensm Unters Forsch 152 (1973) 145


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