400410 Separation of free and conjugated bile acids using normal phase TLC.

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Separation of free and conjugated bile acids using normal phase TLC.

Summary

Type: TLC
Application #: 400410
Catalog#: 1615026 - Silica G TLC Plates, glass backed, 250um, 20x20cm 25/pk
Detection: liquid scintillation counting
Conditions: Eluents :
H: chloroform / methanol / water (70:25:3, v/v)
1. isooctane / isopropanol / acetic acid (60:40:1, v/v)
2. n-butanol / acetic acid / water (100:7:5, v/v)
3. isooctane / isopropyl ether / acetic acid (50:25:50, v/v)
4. toluene / acetic acid / water (50:50:10, v/v)
5. isooctane / acetic acid / isopropyl ether / isopropanol (10:6:5:1, v/v)
6. acetic acid / carbon tetrachloride / isopropyl ether / isoamyl acetate /
n-propanol / benzene* (5:20:30:40:10:10, v/v)

(* instead of benzene we recommend the less toxic toluene)
Authors: Huang CTL
Source: J Chromatogr 109 (1975) 427


Details


Legend: free bile acids: lithocholic acid (LC) deoxycholic acid (DOC) chenodeoxycholic acid (CDC) cholic acid (C) ursodeoxycholic acid (UrsoDOC) 7-ketodeoxycholic acid (7-ketoDOC) hyocholic acid (HyoC)