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Separation of bile acids using normal phase TLC
Summary
Type: TLCApplication #: 400400
Catalog#: 2115126 - Silica Gel HL TLC Plates, w/UV254, glass backed, 250um, 20x20cm 25/pk
Detection: 0.003% MnCl2 in H2O / MeOH / H2SO4, 30:30:2 (v/v), 100-120 C, TLC scanner, UV 365 nm
Conditions: Eluent: iso-octane / dibutyl ether / acetic acid (40:25:25, v/v) Migration distance: 8 cm/40 min
Authors: TLC department
Source: TLC department
Details
Legend: Components: (0.13% per component) 1. Hyocholic acid 2. a-Hyodeoxycholic acid 3. Chenodeoxycholic acid 4. Lithocholic acid