Summary
Type: TLC
Application #: 400110
Catalog#: 2514156 - C18 Silica HPTLC Plates, w/UV254, 100% silanization, glass backed, 200um, 10x10cm 25/pk
Detection: dry plates 2 min at 140 °C and place 15 min at ambient temperature in chamber with iodine vapour (dark brown spots). For very low concentrations heat 30 min to 140 °C and view under UV at 366 nm (brig
Conditions: Eluent: acetone / triethylamine / water (90:6:4, v/v), chamber saturation
Authors: Ebner T
Source: Ther Drug Monit 11 (1989) 214 - 216
Details
Legend: TLC separation of pure reference samples of
Sparteine = SP (a)
2,3-Didehydrosparteine = 2-DHSP (b)
5,6-Didehydrosparteine = 5-DHSP (c),
40 nmol/ml, 4 µl/spot and of urine extracts from EM