Contact an Account ManagerAlkaloids
Summary
Type: TLC
Application #: 400100
Catalog#: 0314126 - Cellulose 300 TLC Plates, w/UV254, glass backed, 100um, 20x20cm 25/pk
Detection: UV 254 nm
For impregnation dip plate in 20% formamide solution in acetone and dry 5 min in a cold air stream. Water-soluble ergot alkaloids remain at the starting line.
Conditions: Eluent: 2-fold development in the same direction:
1. benzene* / heptane / chloroform (6:5:3, v/v),
2. benzene* / heptane (6:5, v/v)
Migration distance: 15 cm each in about 40 min
* instead of benzene we recommend the less toxic toluene
Authors: Teichert K
Source: Deut Apotheker-Ztg 100 (1960) 283
Details
Legend: Separation of water-insoluble ergot alkaloids on formamide impregnatedcellulose layers:ErgotamineErgosineErgotaminineErgocristineEergocornineErgocriptineErgocristinine