Summary
Type: SPE
Application #: 305820
Catalog#: 730747 - SPE Cartridges, Chromabond, HR-XAW, 3mL, 60mg, 30/pk
Detection:
Conditions: Column conditioning: 2 mL 0.1 % methanol, then 2 mL methanol, at last 2 mL water
(Do not let run the column dry.)
Sample aspiration: the pretreated sample is passed through the column by vacuum (max. 5 mL/min)
Washing: 2 mL water, then 2 mL 1 % formic acid in acetone – acetonitrile (1:1, v/v), at last 2 mL methanol
Drying: no
Elution: 3 x 0.8 mL 0.1 % ammonia in methanol (max. 2 mL/min)
Evaporation: to dryness (nitrogen, 40 °C)
Reconstitution: 0.5 mL methanol – water (6:4, v/v)
Authors: Normenausschuss Wasserwesen (NAW) im DIN
Source: DIN 38407-42:2011-03