Summary
Type: SPE
Application #: 305490
Catalog#: 730934 - SPE Cartridges, Chromabond, HR-X, 1ml, PP, 30mg, 30/pk
Detection:
Conditions: To determine the different elution behavior hexobarbital was enriched on both columns as follows:
First, both columns were conditioned with 5 mL of methanol and 5 mL of water.
Then 1 mL sample solution was given onto each of the columns.
Next the columns were washed with 5 mL of water.
After drying the columns, methanol was given onto the column in 100 µL steps.
The eluates of every step were collected and the solvent was evaporated to dryness.
At last the samples were reconstituted in HPLC eluent and analyzed separately by HPLC.
Authors: SPE department
Source: Macherey-Nagel, Germany 2011