Summary
Type: SPE
Application #: 305340
Catalog#: 730735 - SPE Cartridges, Chromabond, HR-XCW, 3mL, 60mg, 30/pk
Detection:
Conditions: Column conditioning: 3 ml methanol, then 3 ml water
(Do not let the column run dry!)
Sample aspiration: 250 µl spiked serum, diluted with 1 ml 10 % formic acid in water
Washing: 1 ml 5 % formic acid in water, then 1 ml methanol
Drying: 15 min with vacuum
Elution: 3 ml 5 % formic acid in methanol
Evaporation: to dryness
Reconstitution: with a suitable solvent
Authors: SPE department
Source: Macherey-Nagel, Germany 2010