Summary
Type: SPE
Application #: 305290
Catalog#: 730934 - SPE Cartridges, Chromabond, HR-X, 1ml, PP, 30mg, 30/pk
Detection:
Conditions: Column conditioning:
1 mL acetonitrile followed by 1 mL de-ionized water (0.1 % TFA)
Sample application:
Slowly force or aspirate the sample through the column
Column washing:
2 x 1 mL de-ionized water (0.1 % TFA)
Elution:
250 µL of
40:60 ACN/water (acidic peptides)
70:30 ACN/water (neutral peptides)
70:30 ACN/1.0 % formic acid (basic peptides)
Authors: SPE department
Source: Macherey-Nagel (Germany 2009)