Summary
Type: SPE
Application #: 305260
Catalog#: 730061 - SPE Cartridges, Chromabond, Cyano, 1ml, PP, 100mg, 100/pk
Detection:
Conditions: Column conditioning: 10 % isopropanol/0.1 % trifluoro acetic acid
(Do not let run the column dry!)
Sample aspiration: The prepared sample was loaded.
Washing: With 2 ml water
Elution: Step-gradient (10-100 % isopropanol/0.1 % trifluoro acetic acid) elution of the complex was carried out and the eluted fractions (1ml pro isopropanol concentration) were obtained.
Concentration of these fractions using speed vac. concentrator
Authors: Shallan, M.A.
Source: Arab Journal of Biotechnology, Vol 2. No (1)