305220 Foodstuffs - Simultaneous determination of nine sweeteners

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Foodstuffs - Simultaneous determination of nine sweeteners

Summary

Type: SPE
Application #: 305220
Detection:
Conditions: Column conditioning: 3 mL methanol, then 3 x 2 mL of buffer solution (see above); Do not let run the column dry! Sample aspiration: 2 x 5 mL of the prepared sample is passed through the column by vacuum (flow rate of 1–2 mL/min) Washing: With 3 mL buffer solution (see above) are washed impurities Elution: Elute the sweeteners with 2 mL methanol (flow rate: 1 mL/min); wait 10 min before applying a second portion of 2 mL of methanol in the same 5 mL test tube. Evaporate the solvent to 3 mL under a stream of nitrogen at ambient temperature.
Authors: Wasik, A.; Buchgraber, M.
Source: European Comission, Joint Research Centre