Contact an Account ManagerAutomated method for mixed phase-SPE of acidic, neutral and basic compounds from serum
Summary
Type: SPE
Application #: 305090
Catalog#: 730168 - SPE Cartridges, Chromabond, Drug, 3ml, PP, 200mg 50/pk
Detection:
Conditions: Automated SPE method on Zymark Rapid Trace SPE Workstation 1.20:
Column conditioning:
2.0 ml methanol, 2.0 ml/min
2.0 ml 0.1 M phosphat buffer, pH 6, 2.0 ml/min
Sample aspiration:
3.0 ml prepared sample, 0.5 ml/min
Washing 1:
2.0 ml 0.1 M acetic acid, 1.0 ml/min
Drying 1:
nitrogen, 1 min
Washing 2:
0.1 ml methanol, 2.0 ml/min
Drying 2:
nitrogen, 1 min
Elution 1 (acidic/neutral fraction):
1.5 ml acetone/dichloromethane (1:1, v/v), 1.0 ml/min
Drying 3:
nitrogen, 1 min
Elution 2 (basic fraction):
1.5 ml dichloromethane/iso-propanol/25 % amonia solution (80:20:2, v/v/v), 1.0 ml/min
Cleaning of hollow needle:
4.0 ml methanol, 30 ml/min
Authors: Müller, C.
Source: Dissertation, Albert-Ludwigs-Universität Freiburg