303920 Automated SPE for Analysis of Antibiotics in Food

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Automated SPE for Analysis of Antibiotics in Food

Summary

Type: SPE
Application #: 303920
Catalog#: 730013 - SPE Cartridges, Chromabond, C18 ec, 3ml, PP, 500mg, 50/pk
Detection: LC/MS with RP column (e.g. EC 250/2 NUCLEODUR® C18 Gravity, 5 µm, MN Cat. No. 760100.20): Mobile phase: Eluent A: 0.005 M ammonium formiat / NH3, pH 8.5; Eluent B: acetonitrile; Gradient: 25% B for 1
Conditions: Column conditioning: 4 ml methanol, then 4 ml water

Sample application: Applying of 4 ml sample extract

Column washing: 1 ml water, then 4 ml methanol / water (1:10)

Elution: 3 ml methanol / water (1:1).
Authors: Helle N TeLA GmbH Bremerhaven Germany
Source: Labor Praxis April 2006 52-55


Details


Legend: Recovery rates (chloramphenicol): Automation: 92.1% +/- 2.0% Manual: 89.6% +/-2.2% Detection limit (chloramphenicol in prawn): LC/MS (Single Quad System): 0.05 µg/kg LC/MS (Iontrap System): 0.0