303880 Extraction of THC and 11-nor-9-THC-carboxylic acid from urine

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Extraction of THC and 11-nor-9-THC-carboxylic acid from urine

Summary

Type: SPE
Application #: 303880
Catalog#: 730680 - SPE Cartridges, Chromabond, Drug II, 3ml, PP, 200mg, 50/pk
Detection: For further analysis we recommend GC-MS on an OPTIMA® 5 MS (e.g. Cat. No. 726220.30) column after derivatisation with 50 µl Silyl-991 (Cat. No. 701480; BSTFA –TMCS 99:1) at 70°C / 20 min – inject 1-2
Conditions: Column conditioning: 2 ml methanol, followed by 2 ml distilled water – equilibrate column with 2 ml 50 mM ammonium acetate buffer

Sample application: slowly force or aspirate the sample through the column (1-2 ml per min)

Column washing: interferences are eluted with 10 ml methanol – water (1:1, v/v); the column is dried for 10 min at high vacuum; the column is further washed with 2 ml acetonitrile and dried for another 2 min

Elution: THC metabolites are eluted with 3 ml hexane – ethyl acetate – glacial acetic acid (75:25:1, v/v/v)
Authors: SPE department
Source: Macherey-Nagel


Details


Legend: Recovery rates: 70 - 80%