303320 Leukotrienes and other eicosanoids in biological samples from asthmatic subjects

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Leukotrienes and other eicosanoids in biological samples from asthmatic subjects

Summary

Type: SPE
Application #: 303320
Catalog#: 730002 - SPE Cartridges, Chromabond, C18, 3ml, PP, 200mg, 50/pk
Detection: HPLC analysis with EC 250/4.6 NUCLEOSIL® 100-5 C18 in reference to Appl. No. 118660
Conditions: Column washing: The columns were washed with 2 ml water and 2 ml 50% methanol.

Elution: Leukotrienes were finally eluted with 2 ml pure methanol.

Evaporation: Methanol eluates were taken to dryness under a gentle stream of nitrogen.
Authors: Kumlin M
Source: Journal of Chromatography A 725 (1996) 29-40


Details


Legend: Recovery rates The absolute recovery of domperidone from human plasma using this method was 83+/- 2,17%. The mean recovery of 0,2 µg/ml was 97,55+/-4,38% (n=6).