303050 Serum Extraction Methods for Systematic Toxicological Analysis with Mass Spectra Libraries

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Serum Extraction Methods for Systematic Toxicological Analysis with Mass Spectra Libraries

Summary

Type: SPE
Application #: 303050
Catalog#: 730168 - SPE Cartridges, Chromabond, Drug, 3ml, PP, 200mg 50/pk
Detection: 20 µl of each extract was injected into the LC-MS system.
Conditions: Column conditioning: with 2 ml MeOH and 2 ml KH2PO4

Column washing: The column was rinsed with 1 ml 0,1 M HAC and 0,1 ml MeOH.

Elution: The first fraction (acidic/neutral fraction) was eluted by 1,5 ml acetone-dichloromethane (1:1, v/v). Then the column was eluted with 1,5 ml of a mixture of dichloromethane-2-propanol-NH4OH 25% (80:20:2, v/v/v) to obtain the alkaline fraction.

Evaporation: The eluates were evaporated to dryness and were redissolved with 100 μl each (A-B, 1:1, v/v).
Authors: Müller C Schäfer P Störtzel M Vogt S Weinmann W
Source: Journal of Chromatography B 773 (2000) 47-52


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