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Solid-phase extraction of polar hydrophilic aromatic sulfonates
Summary
Type: SPEApplication #: 302740
Catalog#: 730108 - SPE Cartridges, Chromabond, HR-P, 3ml, PP, 200mg 30/pk
Detection: Liquid chromatography-UV-DAD-mass spectrometry
Conditions: Column conditioning: The adsorbents were activated and conditioned first with 7 ml methanol and after that with 3 ml water (acidified to pH 2,5 with sulphuric acid) at a flow-rate of 1 ml/min.
Sample application: The sorbents were not allowed to dry , and subsequently different volumes of spiked water samples were passed through the cartridge at a flow rate of 5 ml/min. After passing the water samples, the cartridges were not allowed to run dry.
Elution: The compounds were eluted into glass vials, first with 1 ml water containing 5 mM TEA-acetic acid and then with 6 ml of methanol-acetone (1:1, v/v).
Evaporation: The organic methanol-acetone solvent was evaporated under a gentle stream of nitrogen until the 1 ml water was left. If necessary, (if some water had been evaporated) the vials were filled up with water (containing 5 mM TEA-5 mM acetic acid) to a final volume of approximately 1 ml (the overall enrichment factor by the extraction of 150 ml water was 150).
Authors: Loos R Alonso M C Barcelo D
Source: Journal of Chromatography A 890 (2000) 225-237
Details
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