302440 Investigation of proanthocyanidins by HPLC with electrospray ionization mass spectrometry

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Investigation of proanthocyanidins by HPLC with electrospray ionization mass spectrometry

Summary

Type: SPE
Application #: 302440
Catalog#: 730127 - SPE Cartridges, Chromabond, Polyamide 6, 6ml, PP, 1000mg, 30/pk
Detection: HPLC-UV-ESI-MS analysis
Conditions: Column conditioning: Polyamide CC 6 (500 g) was shaken with 3,35 l deionised water and 625 ml methanol in a 5 l-plastic container. After overnight precipitation of the polyamide the supernatant was removed, 625 ml methanol added again and the procedure repeated.
A glass column (20 x 1,4 cm i.d.) was filled with cotton wool, sea sand, swollen polyamide (high 6 cm) and sea sand. The polyamide was washed methanol-free with 100 ml deionised water.

Sample application: The concentrated extract was applied to the top of the column.

Column washing: The flask was rinsed twice with 5 ml deionised water in total, and then the column was washed with 25 ml water.

Elution: The polyphenols were eluted in three steps: 30 ml methanol (fraction M1), 90 ml methanol (fraction M2) and 40 ml DMF (fraction D)(approximately 60 drops/min).

Evaporation: Fraction M1 and fraction M2 were concentrated to about 1 ml, and fraction D evaporated to dryness. All residues were transferred to a 5 ml flask with acetic acid (2%):acetonitrile (98:2, v/v), filled up with the same solution and filtered through a 0,2 μm PTFE filter (polytetrafluorethylene).
Authors: Friedrich, W.; Eberhardt, A.; Galensa, R.
Source: Eur. Food Res. Technol. 211 (2000), 56–64


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