301170 Drugs: Opiates from Blood and Serum

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Drugs: Opiates from Blood and Serum

Summary

Type: SPE
Application #: 301170
Catalog#: 730168 - SPE Cartridges, Chromabond, Drug, 3ml, PP, 200mg 50/pk
Detection:
Conditions: Column conditioning:
5 ml methanol, 5 ml water and 5 ml borate buffer 0.05 M, pH 8.5

Sample application:
Force or aspirate the clear supernatant from the sample pretreatment through
the column in about 5 min

Column washing:
2 ml dist. water, 1 ml acetate buffer 0.1 M, pH 4.0, then 2 ml methanol; dry
column under vacuum for 5 min

Elution:
Elute with freshly prepared dichloromethane – isopropanol – concentrated
ammonia (80:20:2, v/v/v) under gravity force.

For further analysis we recommend GC-MS on an OPTIMA® 1 fused silica column (10
m x 0.25 mm ID), temperature programme 1 min at 150 °C, 20 °C/min to 250 °C, 5
min at 250 °C or HPLC on a 250 x 4.6 mm ID column NUCLEOSIL® 100-5 C18 AB,
eluent acetonitrile – 5 mM citrate buffer pH 5.0 containing 20 mM Li
perchlorate (12:88, v/v), flow rate 0.8 ml/min
Authors: Bogusz MJ
Source: J Chromatogr 683 (1996) 177 - 188


Details


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