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Beta-blocker: atenolol from plasma
Summary
Type: SPEApplication #: 300960
Catalog#: 730061 - SPE Cartridges, Chromabond, Cyano, 1ml, PP, 100mg, 100/pk
Detection:
Conditions: Column conditioning:
2 x 1 ml acetonitrile, then 2 x 1 ml dist. water
Sample application:
Slowly force or aspirate 1 ml plasma through the column
Column washing:
500 μl water, then 500 μl acetonitrile
Elution:
2 x 250 μl 0.05 M disodium hydrogen phosphate / acetonitrile (7 : 3, v/v) with
4 mM triethylamine, adjusted to pH 4 with orthophosphoric acid
Authors: Macherey-Nagel
Source: LC department
Details
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