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Antineoplastic agents: methotrexate from plasma
Summary
Type: SPEApplication #: 300900
Catalog#: 730011 - SPE Cartridges, Chromabond, C18 ec, 1ml, PP, 100mg, 100/pk
Detection:
Conditions: Column conditioning:
5 ml methanol, then 5 ml dist. water
Sample application:
Slowly force or aspirate the sample through the column
Column washing: 2 ml 10 mM ammonium formiate buffer (pH 3.5) containing 1 g /
l ascorbic acid
Elution:
Acetonitrile – 1 g / l ascorbic acid in 10 mM phosphate buffer pH 6 (5 : 95,
v/v)
Authors: Macherey-Nagel
Source: LC-department
Details
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