300780 Antibiotics: cyclosporine from blood

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Antibiotics: cyclosporine from blood

Summary

Type: SPE
Application #: 300780
Catalog#: 730013 - SPE Cartridges, Chromabond, C18 ec, 3ml, PP, 500mg, 50/pk
Detection:
Conditions: Column conditioning:
2 column volumes ethanol / dist. water (95 : 5, v/v), then 1 column volume
dist. water

Sample application:
Slowly force or aspirate 1 ml pretreated blood sample through the column

Column washing:
5 ml acetonitrile / dist. water (1 : 1, v/v)

Elution:
250 μl ethanol / water (95 : 5, v/v); then mix the eluate with 200 μl water and
500 μl n-hexane and centrifuge: cyclosporin is in the lower phase
Authors: Macherey-Nagel
Source: LC department


Details


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