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Polyethylene Glycol 400 from Plasma/Urine
Summary
Type: SPEApplication #: 300490
Catalog#: 730003 - SPE Cartridges, Chromabond, C18, 3ml, PP, 500mg, 50/pk
Detection:
Conditions: Column conditioning:
force or aspirate 5 ml methanol, then 5 ml dist. water/methanol (99:1, v/v)
through the column
Sample application:
apply sample to the column; rinse sample vessel with dist. water/methanol
(99:1, v/v) and add rinsing liquid to the column; slowly force or aspirate the
sample through the column
Column washing:
force or aspirate 4 ml dist. water/methanol (99:1, v/v), then 4 ml hexane
through the column
Elution:
10 ml dichloromethane/isopropanol (85 : 15, v/v); evaporate in a gentle stream
of nitrogen and redissolve with 200 μl toluene and 50 μl heptafluorobutyric
acid anhydride (HFBA)
Authors: Fakt C Ervik M
Source: J Chromatogr B 700 (1997) 93 - 100
Details
Legend: