300280 Fatty acids cholesterol and bile acids from tissue

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Fatty acids cholesterol and bile acids from tissue

Summary

Type: SPE
Application #: 300280
Catalog#: 730079 - SPE Cartridges, Chromabond, SB, 3ml, PP, 500mg, 50/pk
Detection:
Conditions: Conditioning of the C18 ec column:
2 column volumes methanol, then 2 column volumes dist. water

Sample application:
Slowly force or aspirate the pretreated sample through the conditioned column

Column washing:
1 ml water / acetone, 8 : 2, v/v (reduce volume, if acids or lipids are
eluted); then dry the column under vacuum for 3 min

Elution:
Elute cholesterol and fatty acids with 2 x 500 μl petroleum ether (fraction 1),
then elute the bile acids with 2 x 500 μl diethyl ether

Conditioning of the SB column:
2 column volumes petroleum ether

Sample application:
Apply cholesterol and fatty acid fraction (fraction 1) to the conditioned SB
column and collect the eluate

Column washing:
1 – 2 ml petroleum ether, combine this eluate with the eluate from the Sample
application: cholesterol fraction

Elution:
Elute fatty acids with 2 x 500 μl acetonitrile / 0.1 M dipotassium hydrogen
phosphate buffer pH 3 (7 : 3, v/v)
Authors: SPE department
Source: Macherey-Nagel


Details


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