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Anthocyanines carbohydrates org. acids from wine
Summary
Type: SPEApplication #: 300160
Catalog#: 730013 - SPE Cartridges, Chromabond, C18 ec, 3ml, PP, 500mg, 50/pk
Detection:
Conditions: Column conditioning:
Place C18 ec column on top of the SB column using the adaptor; condition with 2
column volumes methanol followed by 2 column volumes dist. water
Sample application:
Slowly force or aspirate 1 ml wine through the columns and collect the eluate
Column washing:
3 ml water, combine the eluates: carbohydrate fraction
Elution:
Separate columns, elute the C18 ec column with 2 ml 1 % hydrochloric acid in
methanol: anthocyanine fraction;
elute the SB column with 3 ml 1 N hydrochloric acid: organic acids
Authors: Macherey-Nagel
Source: LC Department
Details
Legend: