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Elution of substances from fat-containing samples
Summary
Type: SPEApplication #: 300020
Catalog#: 730014 - SPE Cartridges, Chromabond, C18 ec, 6ml, PP, 500mg, 30/pk
Detection:
Conditions: Column conditioning:
1 column volume methanol, then 1 column volume dist. water
Sample application:
Slowly force or aspirate 0.5 ml of the clear supernatant through the column
Column washing:
0.5 ml acetonitrile / water (7 : 3, v/v); dry column under vacuum for 5 min
Elution:
some ml of a nonpolar solvent (e.g. n-hexane)
Authors: Macherey-Nagel
Source: LC-department
Details
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