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Removal of Chlorophyll from Plant Cells
Summary
Type: SPEApplication #: 300010
Catalog#: 730077 - SPE Cartridges, Chromabond, SA, 3ml, PP, 500mg, 50/pk
Detection:
Conditions: Column conditioning:
Place 0.5 g dry Na2SO4 onto the SA packing and condition column with 1 column
volume dichloromethane / acetone (3 : 1, v/v) (special columns containing 500
mg CHROMABOND® SA and 500 mg Na2SO4 are available on request)
Sample application:
Slowly force or aspirate 4 ml sample through the column and collect the
chlorophyll-free eluate. When chlorophyll is eluted, reduce the sample volume
1. Elution:
0.5 ml dichloromethane / acetone (3 : 1, v/v), combine the eluate with the
chlorophyll-free eluate
2. Elution:
If the substance to be determined still remains on the column together with the
chlorophyll, elute the desired compounds with a more polar eluent (acetone,
acetonitrile, methanol) or an eluent of lower pH value
Authors: Macherey-Nagel
Source: LC-department
Details
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