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Methylation with TMSH
Summary
Type: GCApplication #: 213060
Catalog#: 701520.101 - TMSH. 0.2 M. 10x1 ml
Detection: GC; For GC separation of FAMEs from natural butter fat after derivatisation with TMSH see Appl. No. 201680
Conditions: Methylation with TMSH [W. Butte, J. Chromatogr. 261 (1983)
142] is recommended for free acids, chlorophenoxycarboxylic
acids, their salts and derivatives as well as for
phenols and chlorophenols. One great advantage is simplification
of the sample preparation. Lipids or triglycerides
can be converted to the corresponding fatty acid
methyl esters (FAMEs) by a simple transesterification.
Isomerisations of multiple unsaturated fatty acids have
not been observed.
This reaction is very elegant and convenient, because it
is just necessary to add the reagent (0.2 M in methanol)
to the sample solution. Removal of excess reagent is
not required, since in the injector of the gas chromatograph
at 250 °C pyrolysis to volatile methanol and
dimethylsulfide will occur. Due to the high reactivity,
complete derivatisation is often obtained at ambient
temperature. However, heating (e. g. 10 min at 100 °C)
in a closed sample vial may be necessary.
Authors: Macherey-Nagel
Source: GC department (2007)
Details
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