125210 Separation of four nucleosides

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Separation of four nucleosides

Summary

Type: HPLC
Application #: 125210
Catalog#: 763332.40 - MN HPLC Column, Nucleoshell Hilic, 90A, 2.7um, 4x50mm
Detection:
Conditions: Eluent A: acetonitrile Eluent B: 100 mmol/L ammonium acetate, pH 5.35 Gradient: 92.5 % A for 2 min , to 50 % A in 5.5 min Flow rate: 1.3 mL/min Temperature: 25 °C Injection volume: 1.0 µL
Authors: HPLC department
Source: Macherey-Nagel, Germany 2011