124930 Comparison of the separation of steroids on NUCLEOSHELL RP 18 and on NUCLEODUR

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Comparison of the separation of steroids on NUCLEOSHELL RP 18 and on NUCLEODUR

Summary

Type: HPLC
Application #: 124930
Catalog#: 763132.30 - MN HPLC Column, Nucleoshell RP 18, 90A, 2.7um, 3x50mm
Detection:
Conditions: NUCLEOSHELL RP 18 NUCLEODUR C18 Gravity Eluent A: acetonitrile acetonitrile Eluent B: water water Gradient: 30–80 % A in 1.0 min, hold for 0.5 min 30–80 % A in 5.0 min, hold for 2.0 min Flow: 2.0 mL/min 1.0 mL/min Temperature: 25 °C 25 °C Pressure: 350 bar 280 bar Injection volume: 1.0 µL 1.0 µL
Authors: HPLC department
Source: Macherey-Nagel, Germany 2011