124840 Separation of β-lactam antibiotics with different buffers on NUCLEODUR® PFP

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Separation of β-lactam antibiotics with different buffers on NUCLEODUR® PFP

Summary

Type: HPLC
Application #: 124840
Catalog#: 760458.30 - MN HPLC Column, Nucleodur, PFP, 110A, 5um, 3x150mm
Detection:
Conditions: Eluent A: acetonitrile acetonitrile Eluent B: 25 mmol/L KH2PO4, pH 2.7 0.1 % trifluoroacetic acid in water Gradient: 10-60 % A in 15 min 10-45 % A in 15 min, hold for 5 min Flow: 0.563 mL/min 0.563 mL/min Temperature: 40 °C 50 °C Injection vol.: 1.0 µL 1.0 µL
Authors: HPLC department
Source: Macherey-Nagel, Germany 2011