124830 Separation of tricyclic antidepressants at pH 3.0 and pH 7.0 on NUCLEODUR

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Separation of tricyclic antidepressants at pH 3.0 and pH 7.0 on NUCLEODUR

Summary

Type: HPLC
Application #: 124830
Catalog#: 760459.40 - MN HPLC Column, Nucleodur, PFP, 110A, 5um, 4x250mm
Detection:
Conditions: Eluent 1: acetonitrile – 20 mmol/L KH2PO4, pH 3.0 (24:76, v/v) Eluent 2: acetonitrile – 20 mmol/L KH2PO4, pH 7.0 (55:45, v/v) Flow rate: 1.0 mL/min Temperature: 60 °C Injection volume: 1.0 µL
Authors: HPLC department
Source: Macherey-Nagel, Germany 2011