124590 Separation of preservatives

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Separation of preservatives

Summary

Type: HPLC
Application #: 124590
Catalog#: 760478.30 - MN HPLC Column, Nucleodur, PolarTec, 110A, 3um, 3x150mm
Detection:
Conditions: Eluent A: acetonitrile with 0.1 % TFA Eluent B: water with 0.1 % TFA Gradient: 20 % A -> 50 % A in 12 min -> 65 % A in 2 min -> 95 % A in 6 min Flow rate: 0.9 mL/min Temperature: 45 °C Injection volume: 5.0 µL
Authors: HPLC department
Source: Macherey-Nagel, Germany 2011